Cloning and Characterization of Two Inhibitors of Apoptosis (IAP) from Eriocheir hepuensis and Their Expression Pattern in the Immune Organs under Azadirachtin Stress

Inhibitors of apoptosis proteins (IAPs) maintain a balance between cell growth and death by suppressing caspase activity modulating immunological responses. Here, two IAPs (EhIap1 EhIap2) were identified from Eriocheir hepuensis using RT-PCR technology characterized bioinformatics software. Sequence analysis showed that the open reading frames EhIap1 EhIap2 composed 1,839 bp 696 encoded 612 amino acids 231 acids, respectively. contained BIR domains, one UBA domain, RING only domains. qRT-PCR expressed in various tissues, their expression levels highest hepatopancreas. Expression EhIAP1 Eh1ap2 more than 87.09 411 times higher hepatopancreas muscle, Under azadirachtin stress, was increased three immune tissues (hepatopancreas, gill, heart). The 18.44, 17.75, 6.16 hepatopancreas, heart, gill under stress compared with control group, 15.48, 3.91 heart tissue surviving individuals dead individuals. Overall, genes first cloned, our findings indicate EhIAPs might be involved E. against through regulating apoptosis.